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Attomole-level detection of low-abundance biomarkers using standard ELISA workflows.

Researchers rely on immunoassays for the detection of biomarkers. When test samples contain abundant concentrations of analyte, the process is relatively straightforward. However, the ability to detect biomarkers in low concentrations and distinguish them from background noise has been a limiting factor for many immunoassays.

Exazym® addresses this problem by allowing researchers to reliably detect biomarkers in extremely low concentrations. Unlike other signal amplification methods, Exazym® is an add-on reagent that can be used with existing immunoassays to dramatically lower the limit of detection without the need for expensive equipment or proprietary processes.

The BOLD science behind Exazym®

Binding Oligo Ladder Detection (BOLD)

Exazym® is an add-on reagent based on a new detection method called Binding Oligo Ladder Detection, or BOLD for short. A primer conjugated to the secondary antibody works with a polymerase to create a DNA/RNA hybrid strand. This is how BOLD is able to amplify immunoassay signals by up to 100 times using established science. BOLD technology is broadly applicable for immunoassays and independent of instrument platforms. The extra amplification step can be performed between 20°C to 37°C. This temperature range makes BOLD ideally suited for temperature-sensitive ligands and antibodies.

Transforming translational research

Translational research serves as the vital link between scientific breakthroughs and real-world clinical diagnostics, treatments, and cures. Any significant advance in this field is a win for humanity.

That is why we are so excited about the promise that Exazym holds for translational research globally. Once adopted by kit producers, our BOLD (Binding Oligo Ladder Detection) technology will put cutting-edge immunoassay sensitivity within every researcher’s grasp.

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Order your Exazym® test kits

Exazym® can be used to:
  1. Increase the sensitivity of existing ELISA assays
  2. Increase the sensitivity of ELISA and other assays in development
  3. Test the limits of sensitivity or detect new biomarkers
  4. Improve existing products or products in development
Cavidi service and support includes:
  1. One-on-one customer support to get started
  2. Troubleshooting
  3. Customization of kits if required
  4. Collaboration on new immunoassay development
Order Exazym® online:
  1. Everything you need to get started can be purchased on our web shop right now.
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How to use Exazym®

Exazym® is easy to work with. For example, it works with previously optimized ELISA antibody pairs, standard laboratory equipment, and established testing protocols. Exazym® adds only one additional step to your current workflow. Below is an example using a sandwich ELISA for illustration. If needed, our specialists can work with you to optimize Exazym® for your immunoassay.

Download the Exazym® data sheet

Learn how our BOLD technology delivers, how it’s designed to achieve high sensitivity, and is ready for the future. See a comparison of the results applying BOLD to a standard ELISA, and other applications.

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Learn more about Exazym® and BOLD

Download our new White Paper for:

  • Our latest results with human anti-spike IgG antibodies in serum samples and spike-antigen assessment (both key biomarkers related to Sars-Cov-2)
  • The science behind our patented BOLD signal amplification technology
  • Details on how you can get started with Exazym®
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Free consultation for kit providers

Contact us for a no-obligation technical expert consultation to discuss how Exazym® could be integrated into your immunoassay for signal enhancement.

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