Extend the Lower Limit of Detection in Your Existing ELISA Kits — No Assay Redesign
Extend the Lower Limit of Detection in Your Existing ELISA Kits — No Assay Redesign
Detect far lower concentrations in a commercial sandwich ELISA you already run. Exazym® StreptaClick® Primer clicks onto the biotinylated detector antibody that’s already in your kit and typically lowers its detection limit by 10–100× (up to ~200× under optimal conditions), with no chemical modification and no re-validation from scratch.
Download the White Paper
~8×
Lower detection limit for IL‑4 in this feasibility study
80–120%
Spike recovery maintained across the tested range
0
Assay redesigns required to implement
Your kit has a sensitivity floor. Redesigning it is expensive.
Commercial ELISA kits are validated and reliable, but capped at a fixed limit of detection. Pushing sensitivity lower usually means re-engineering the assay, sourcing new reagents, and re-running your entire validation. Most labs would rather avoid that cost, time, and risk.
Exazym® StreptaClick® Primer takes a different route.
Most commercial kits already use a biotinylated detector antibody. Exazym® StreptaClick® Primer clicks straight onto that biotin and adds an amplification layer built on Exazym®’s BOLD (Binding Oligo Ladder Detection) technology, which multiplies the signal from each captured target. You can measure far lower concentrations using the reagents and workflow you already have.
How Exazym® StreptaClick® Primer works
A standard sandwich ELISA runs exactly as it does today, right up to the biotinylated detector antibody (step 3). From there, Exazym® StreptaClick® Primer adds just three BOLD-specific steps (4–6), using reagents you already have, to build an amplified detection ladder from a single binding event.
Add BOLD to the ELISA you already run.
Exazym® StreptaClick® Primer pairs with the Exazym® Polymerase Reaction and Biotin Detection kits to bring BOLD amplification to the ELISA you already run. No new detector antibody to source, no covalent conjugation step, no re-validation from the ground up
Recovery of spiked IL-4 in diluent
What's inside the white paper
Across analytes, Exazym®’s BOLD technology typically delivers a 10–50× lower detection limit, and up to ~200× under optimal conditions. This paper documents a conservative ~8× for IL‑4 in human plasma, from a single, minimally optimized run.
- A working model ELISA for human IL-4, a low-abundance cytokine and clinically relevant target.
- How to optimize detector-antibody and Exazym® StreptaClick® Primer concentrations for clean amplification.
- Head-to-head performance vs. the standard ELISA on the same samples.
- Dilution linearity and spike recovery in human plasma.
- A step-by-step assay protocol you can adapt to your own biomarker.
Get the full study and protocol
See the data behind Exazym® StreptaClick® Primer and the step-by-step method for adding BOLD to your own biotinylated ELISA. You’ll get the full 9-page PDF with methods, data tables, and protocol.
