From Buffer to Plasma: Ultrasensitive IL‑4 Detection with Exazym® StreptaClick® Primer

On-Demand Webinar

From Buffer to Plasma: Ultrasensitive IL‑4 Detection with Exazym® StreptaClick® Primer

See how Exazym®’s BOLD (Binding Oligo Ladder Detection) signal amplification performs in human plasma, where it counts. Johanna Hedin walks through the data on sensitivity, dilution linearity, and spike recovery, and how to add it to an ELISA you already run.

Johanna Hedin, Development Engineer
⏱ ~19 minutes
Press play and enter your email once on the player — the full ~19-minute recording plays straight through.

~8×

Lower detection limit for IL‑4 in this feasibility study

9 / 9

Spike recoveries within the 80–120% acceptance band

~19 min

Data-focused session, plus live audience Q&A

What you'll learn

  1. Why IL‑4 in plasma is so hard to measure, and why strong performance in buffer doesn't guarantee it in a biological matrix.
  2. How Exazym® StreptaClick® Primer plugs BOLD amplification into a standard biotinylated ELISA without redesigning the assay.
  3. The single optimization run (just five conditions) used to find working detector-antibody and primer concentrations.
  4. The ~8-fold improvement in limit of detection (0.025 vs. 0.195 pg/mL) and what it means for low-abundance targets.
  5. Plasma compatibility in practice: dilution linearity (89–98%) and spike recovery across three matrices, 9 of 9 within acceptance.
  6. Practical takeaways: what you add, how much time it costs (~70 min, room temperature), and how to tune sensitivity vs. assay time.
Watch the recording
Johanna Hedin Development Engineer, Cavidi
Johanna was part of the team that developed and launched the Exazym® technology. She holds an MSc in Infection Biology and a BSc in Molecular Biology, both from Uppsala University.

Moderated by Jonathan Royce, CEO, Cavidi.

What's covered

A tight, data-led walkthrough from the measurement problem to a plasma-ready result, followed by an extended audience Q&A.

  1. The sensitivity challenge: why IL‑4 in plasma sits at the edge of standard ELISA detection.
  2. The BOLD mechanism and how Exazym® StreptaClick® Primer fits a standard workflow.
  3. Assay optimization: identifying working concentrations in five conditions.
  4. Sensitivity in buffer: the calibration curves and the ~8× LOD gain.
  5. Plasma compatibility: dilution linearity and spike recovery across three matrices.
  6. Practical takeaways (assay time, serum, MSD/sulfo-tag, scalability)

IL-4 calibration curves - BOLD vs. standard ELISA (~8x lower detection limit)

"If you already have a working ELISA and the only issue is sensitivity, you can often add Exazym® StreptaClick® Primer with just one optimization step." — Johanna Hedin, Development Engineer, Cavidi

Watch the full recording

See the data behind BOLD amplification in human plasma and how to apply Exazym® StreptaClick® Primer in your own assay. Enter your email on the player and it plays right away.

Watch the Recording ~19 minutes · presented by Johanna Hedin
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