Key Features:

Based on a highly sensitive, non-radioactive ELISA-technique using 96-well microtiter plate format (2 plates included in the kit)

Measuring range:

0,2 to 400 pg RT/ml

Analyze the activity of the enzyme reverse transcriptase (RT) from lentivirus with this kit. It can be used for quantification or screening for any lentivirus (HIV-1, HIV-2, SIV, FIV and Visnavirus) RT activity in cell culture supernatants.

Another application is screening for potential RT-inhibiting substances, either directly toward RT or in viral cell culture. The Lenti RT Activity Kit is not intended for use as a screening test for HIV-1 infection in humans nor is it to be used as a diagnostic test to confirm the presence of HIV-1 infection in humans.

Small ruminant macrophage polarization may play a pivotal role on lentiviral infection.

Authors: 

Crespo H, Bertolotti L, Juganaru M, Glaria I, de Andrés D, Amorena B, Rosati S, Reina R.

Journal:

Vet Res. 2013 Sep 26;44(1):83. doi: 10.1186/1297-9716-44-83.

Product usage:

For viral infections and RT activity determination, six-day stimulated MDM were infected at 0.1 TCID50/cell with one of the six SRLV strains under study, being one of genotype A (Ev1), three of genotype B (CAEV-Co, CAEV-To and 496) and two of the recently described genotype E of SRLV (Roccaverano and Seui). Following 2-h incubation, the cells were washed three times with PBS and fresh medium containing the stimulating cytokines was added. Supernatants were collected at 7 days post inoculation for Retrotranscriptase activity (RT activity) quantification, performed with the HS-Lenti RT activity kit (Cavidi, Uppsala, Sweden) according to the manufacturer’s instructions.

RT Activity Kits

• Lenti RT Activity kit
• C-type RT Activity kit
• HS-Lenti RT Activity kit
• HS-Mn RT Activity kit
• HS-Mg RT Activity kit