Lenti RT activity kit
High sensitive RT assay
- Visnavirus retroviruses
Based on a highly sensitive, non-radioactive ELISA-technique using 96-well microtiter plate format (2 plates included in the kit)
1 to 2000 pg RT/ml
Analyze the activity of the enzyme reverse transcriptase (RT) from lentivirus with this kit. It can be used for quantification or screening for any lentivirus (HIV-1, HIV-2, SIV, FIV and Visnavirus) RT activity in cell culture supernatants.
Another application is screening for potential RT-inhibiting substances, either directly toward RT or in viral cell culture. The Lenti RT Activity Kit is not intended for use as a screening test for HIV-1 infection in humans nor is it to be used as a diagnostic test to confirm the presence of HIV-1 infection in humans.
Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4+ T-cells.
Chan CH, McMonagle EL, Hosie MJ, Willett BJ
Virus Research. 2013, 171, 121–128
Samples were stored at −80 °C until the end of the experiment at which time virus levels in the culture supernatants were measured by […] a non-isotopic reverse transcriptase activity assay (Lenti RT kit, Cavidi AB, Sweden) as per manufacturer’s instructions
Human serum protein enhances HIV-1 replication and up-regulates the transcription factor AP-1.
Perdomo MF, Hosia W, Jejcic A, Corthals GL, Vahlne A.
PNAS. 2012, 109(43), 17639–17644
PBMC: 200,000 freshly isolated, phytohemagglutinine-stimulated PBMCs were infected with 200 TCID50 of the primary isolate and cultured with human plasma and 200 IU interleukin-2. Control wells were cultured in 10% FBS. All samples were measured in quadruplicates in the same plate and in three different plates. RT was quantified at days 7 and 11 postinfection using the RT activity kit (Cavidi).
RT Activity Kits
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