Where run-to-run variation hides
You’ve built a solid ELISA workflow. The antibody pair is validated, the protocol runs reliably, and the results are reproducible. But even in a well-run assay, every manual preparation step introduces small differences between runs. Run-to-run variation often traces back to exactly that.
Why the conjugation step matters
If you’re running BOLD amplification with the Exazym® ClickChem Conjugation Kit, conjugating the oligo-dT primer to the detector antibody is part of the setup. It works. Researchers using the Exazym® ClickChem Conjugation Kit have been getting consistent, highly sensitive results, because the chemistry is sound.
But it’s still a step — one that takes time and care, and like any hands-on preparation, the result can vary slightly depending on who does it, when, and under what conditions. Operator-to-operator variability is one of the most common sources of inconsistency in manual ELISA workflows, and any step that depends on individual technique is a potential lever.
For most labs, that’s an acceptable trade-off for the sensitivity gains BOLD delivers. For others — high-throughput core facilities, labs running assays across multiple operators, or researchers who simply want one fewer variable — eliminating that step entirely is a real upgrade.
Introducing Exazym® StreptaClick® Primer
Exazym® StreptaClick® Primer is a ready-to-use format of Exazym® built for labs that want BOLD amplification without the offline conjugation step.
It’s a streptavidin-oligo conjugate, pre-formed and quality-controlled before it leaves our lab. You add it directly to your standard ELISA workflow at the detection step. No offline chemistry, no preparation protocol, and the same consistent starting point every run, regardless of who’s at the bench.
Designed for standard sandwich ELISA workflows that use a biotinylated detector antibody — or one that can be adapted to use one — it gives researchers coming to BOLD amplification for the first time a direct route from their existing ELISA. It’s also a natural fit for established labs that want to reduce operator-to-operator variability without redesigning their workflow.
For labs that need full flexibility over detector conjugation, including non-standard formats, the Exazym® ClickChem Conjugation Kit is the right choice. And for specific cytokine targets, the Exazym® Antibody Pair Kits offer a fully preconfigured option with matched capture and detector antibodies, ready to use.
Who it’s built for
It’s built for labs already running standard sandwich ELISAs who want the most direct path to BOLD amplification. It’s particularly well-suited for:
-
Core facility managers running assays across multiple operators who need consistent results no matter who’s at the bench
-
Researchers new to BOLD amplification who want a direct path from their existing ELISA
-
Lab directors and PIs who want reproducibility built in rather than dependent on individual technique
If you’re already running BOLD amplification with the Exazym® ClickChem Conjugation Kit and your workflow is working well, there’s no need to switch. It remains the right choice when you need full control over detector-antibody conjugation, non-standard assay development, or immunoassay formats beyond standard ELISA. But if you’ve been looking for a direct path from your existing biotinylated ELISA to ultra-sensitive detection, Exazym® StreptaClick® Primer is built for exactly that.
The BOLD performance you already know
Exazym® StreptaClick® Primer uses the same BOLD chemistry as the Exazym® ClickChem Conjugation Kit, the same mechanism that delivers 10–100x sensitivity improvements in most assays and up to 200x in optimal conditions.
What’s coming next
We’ll be going deep on the data and the workflow in our webinar on June 11 — Johanna Hedin, our development engineer, will be walking through the performance data live and taking questions. Register here.
In the meantime, if you’d like to get your ELISA optimized by our team or talk through whether Exazym® StreptaClick® Primer fits your specific assay, we’re happy to help. Reach us at support@cavidi.se.