Built for labs that already run ELISA
Exazym® StreptaClick® Primer is the simplest way to add BOLD signal amplification to a standard sandwich ELISA — the same proven sensitivity gains, without the offline conjugation step. For labs that already trust ELISA, the question isn’t whether the workflow works. It’s how quickly and reliably ultra-sensitive detection can be added to the assays they already run.
That’s why we built Exazym® StreptaClick® Primer — a ready-to-use reagent format based on the same monovalent streptavidin–oligo-dT primer approach shown in our April 2026 cytokine poster, and built on the BOLD method now described in an open-access paper in SLAS Technology (Nordal et al., April 2026). It brings BOLD signal amplification into a standard sandwich ELISA workflow, without the offline conjugation step.
Whether you’ve followed from June 1 or you’re coming to this fresh, everything you need to evaluate Exazym® StreptaClick® Primer is here: what it does, how the early data looks, which Exazym® is right for your workflow, and how to get started.
Why we built Exazym® StreptaClick® Primer
When we talk to researchers running BOLD-amplified ELISAs, the ask is consistent: keep the sensitivity gains, but make it easier for more people on the team to run the assay. For core facilities and multi-operator labs especially, not having an offline conjugation step to teach or optimize makes a real difference from day one — and makes it easier for distributors to recommend with confidence.
So we built it. Exazym® StreptaClick® Primer is a new entry point into BOLD amplification — not a replacement for the Exazym® ClickChem Conjugation Kit, but a format designed for labs that want BOLD without any offline prep work.
What Exazym® StreptaClick® Primer changes
The Exazym® ClickChem Conjugation Kit workflow requires you to conjugate an oligo-dT primer to your detector antibody using the ClickChem Conjugation Kit. It’s a well-documented process and it gives you precise control over the conjugation chemistry — which is exactly why it remains the right choice for researchers who need that flexibility.
Exazym® StreptaClick® Primer removes that step entirely.
It comes as a streptavidin-oligo conjugate — pre-formed at a defined, homogeneous 1:1 ratio and quality-controlled in our lab before it ships to yours. You add it directly to your standard ELISA workflow at the detection step. No offline chemistry. No conjugation protocol. A consistent, standardized reagent input — designed to give teams a reliable starting point for amplified ELISA workflows, regardless of who runs the assay.
Exazym® StreptaClick® Primer is designed for standard sandwich ELISA workflows that use, or can be adapted to use, a biotinylated detector antibody.
Choosing the right Exazym® for your workflow
Both products are built on the same BOLD mechanism. The difference is in workflow and scope.
Exazym® StreptaClick® Primer is built for standard ELISA workflows with a biotinylated detector. For full flexibility over detector conjugation — including non-standard formats — the Exazym® ClickChem Conjugation Kit is the right choice. And for specific cytokine targets, the Exazym® Antibody Pair Kits offer a fully pre-configured option with matched capture and detector antibodies ready to go.
| Choose Exazym® StreptaClick® Primer when… | Choose Exazym® ClickChem Conjugation Kit when… |
|---|---|
| You run standard sandwich ELISAs | You need non-standard or non-ELISA immunoassay formats |
| You want the simplest possible path to BOLD | You want full control over detector-antibody conjugation |
| You need consistency across multiple operators | You are optimizing a specialized or custom assay |
| Your detector antibody is already biotinylated | You need to conjugate a detector antibody of your choice |
If you’re not sure which Exazym® is the right fit, reach out at support@cavidi.se — we’re always happy to talk through the specifics of your assay.
What the performance data shows
Our April 2026 cytokine poster presents results using the same monovalent streptavidin–oligo-dT primer approach behind Exazym® StreptaClick® Primer. In that poster, the approach delivered an 11x improvement in sensitivity for IL-23 (LOD: 9.4 pg/mL vs. 105 pg/mL standard) and a 16x improvement for IL-4 (LOD: 0.52 pg/mL vs. 8.1 pg/mL standard).
For broader context: the original IL-4 application note (click-chemistry method) shows a 50x improvement, from 1.9 pg/mL to 0.040 pg/mL — a custom-optimized assay result, useful context for researchers developing specialized immunoassays rather than a direct comparison with the poster figures above.
Across the broader BOLD evidence base, most assays show 10–50x sensitivity improvements, with up to 200-fold gains under optimal conditions, depending on the assay.
The BOLD method, published in SLAS Technology
The BOLD method is now described in an open-access paper in SLAS Technology (Nordal et al., April 2026). It walks through the core mechanism, the optimization work, and a head-to-head ELISA comparison that showed approximately 70x improved detection sensitivity for SARS-CoV-2 spike protein — published under CC BY-NC-ND 4.0 and freely available to read.
Get started
Start here→ Exazym® StreptaClick® Primer product page
Want the method? → Read the SLAS Technology paper (open access)
Have questions about your specific assay? We’re always happy to help — reach us at support@cavidi.se.