For analyzing the activity of the enzyme RT from lentivirus.
HS-Lenti RT Activity kit
Ultrasensitive RT assay
Based on a highly sensitive, non-radioactive ELISA-technique using 96-well microtitre plate format (2 plates included in the kit)
0,2 to 400 pg RT/ml
Analyze the activity of the enzyme reverse transcriptase (RT) from lentivirus with this kit. It can be used for quantification or screening for any lentivirus (HIV-1, HIV-2, SIV, FIV and Visnavirus) RT activity in cell culture supernatants.
Another application is screening for potential RT-inhibiting substances, either directly toward RT or in viral cell culture. The Lenti RT Activity Kit is not intended for use as a screening test for HIV-1 infection in humans nor is it to be used as a diagnostic test to confirm the presence of HIV-1 infection in humans.
Authors: Crespo H, Bertolotti L, Juganaru M, Glaria I, de Andrés D, Amorena B, Rosati S, Reina R.
Journal: Vet Res. 2013 Sep 26;44(1):83. doi: 10.1186/1297-9716-44-83.
Product usage: For viral infections and RT activity determination, six-day stimulated MDM were infected at 0.1 TCID50/cell with one of the six SRLV strains under study, being one of genotype A (Ev1), three of genotype B (CAEV-Co, CAEV-To and 496) and two of the recently described genotype E of SRLV (Roccaverano and Seui). Following 2-h incubation, the cells were washed three times with PBS and fresh medium containing the stimulating cytokines was added. Supernatants were collected at 7 days post inoculation for Retrotranscriptase activity (RT activity) quantification, performed with the HS-Lenti RT activity kit (Cavidi, Uppsala, Sweden) according to the manufacturer’s instructions.