Lenti RT Activity kit
Lenti RT Activity kit
Lenti RT Activity kit
For analyzing the activity of the enzyme RT from lentivirus.
Lenti RT Activity kit
High sensitive RT assay
Optimized for:
- HIV-1
- HIV-2
- SIV
- FIV
- Visnavirus retroviruses
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DESCRIPTION
CITATIONS & REFERENCES
OTHER RT ACTIVITY KITS
DESCRIPTION
Key Features:
Based on a highly sensitive, non-radioactive ELISA-technique using 96-well microtitre plate format (2 plates included in the kit)
Measuring range:
1 to 2000 pg RT/ml
Analyze the activity of the enzyme reverse transcriptase (RT) from lentivirus with this kit. It can be used for quantification or screening for any lentivirus (HIV-1, HIV-2, SIV, FIV and Visnavirus) RT activity in cell culture supernatants.
Another application is screening for potential RT-inhibiting substances, either directly toward RT or in viral cell culture. The Lenti RT Activity Kit is not intended for use as a screening test for HIV-1 infection in humans nor is it to be used as a diagnostic test to confirm the presence of HIV-1 infection in humans.
CITATIONS & REFERENCES
Authors: Chan CH, McMonagle EL, Hosie MJ, Willett BJ
Journal: Virus Research. 2013, 171, 121–128
Product usage: Samples were stored at −80 °C until the end of the experiment at which time virus levels in the culture supernatants were measured by […] a non-isotopic reverse transcriptase activity assay (Lenti RT kit, Cavidi AB, Sweden) as per manufacturer’s instructions
Human serum protein enhances HIV-1 replication and up-regulates the transcription factor AP-1.
Authors: Perdomo MF, Hosia W, Jejcic A, Corthals GL, Vahlne A.
Journal: PNAS. 2012, 109(43), 17639–17644
Product usage: PBMC: 200,000 freshly isolated, phytohemagglutinine-stimulated PBMCs were infected with 200 TCID50 of the primary isolate and cultured with human plasma and 200 IU interleukin-2. Control wells were cultured in 10% FBS. All samples were measured in quadruplicates in the same plate and in three different plates. RT was quantified at days 7 and 11 postinfection using the RT activity kit (Cavidi).
Selected RT Activity Kits publications, year 2006 to 2013
OTHER RT ACTIVITY KITS