Introducing the Exazym® HRP Detection Kit
You’ve optimized your assay. You’ve validated your antibody pairs. Your protocol works—except for one problem: your capture strategy already uses a streptavidin–biotin reaction, and adding another one later in the workflow creates conflicts.
This is where many researchers hit a wall with signal amplification kits. Traditional approaches rely on a secondary streptavidin–biotin detection step, which doesn’t work if you’ve already committed your streptavidin capacity earlier in the assay.
The problem with traditional approaches
Many labs working with their own antibodies use streptavidin-coated plates or magnetic beads to immobilize the capture antibody. It is a practical, cost-effective approach—until you want to add signal amplification.
Standard Exazym kits use a biotinylated tertiary antibody that recognizes the DNA–RNA hybrid created during BOLD amplification, followed by a streptavidin–HRP conjugate for detection. But if you have already used your streptavidin capacity in the capture step, you are effectively locked out of this detection pathway.
You face a choice: abandon your optimized workflow or abandon ultra-sensitive detection. Neither should be necessary.
How the Exazym® HRP Detection Kit works
The Exazym HRP Detection Kit removes this bottleneck by skipping the streptavidin step entirely.
Instead of relying on biotin–streptavidin interactions for detection, the HRP kit uses a direct HRP-conjugated anti–DNA/RNA antibody. This means:
No streptavidin conflicts. You can use streptavidin–biotin interactions in your capture step without limitation.
One fewer step. After adding your tertiary antibody, you go directly to the plate reader—no additional incubation with streptavidin–HRP.
Same ultra-sensitive results. You retain the sensitivity improvements Exazym delivers; only the detection chemistry has changed to fit your workflow.
Who benefits
The Exazym HRP Detection Kit is designed for researchers who want ultra-sensitive detection without re-engineering their assay:
- Labs using streptavidin-coated plates or magnetic beads for antibody immobilization
- Teams optimizing assays with custom antibody pairs rather than commercial pre-coated plates
- Groups seeking to streamline protocols while maintaining sensitivity and data quality
Real-world impact
By eliminating one incubation step, the HRP kit typically saves 30 minutes per assay run. For labs running multiple plates or high-throughput screens, those minutes translate into more samples processed and fewer workflow bottlenecks.
More importantly, it removes a technical complication from an already complex process, so you can focus on the biology of your experiment rather than the mechanics of your detection strategy.
Compatibility with your existing setup
Like other Exazym products, the HRP Detection Kit is built to integrate into your current workflow, not replace it. It works with:
- Standard ELISA readers—no specialized equipment required
- Your previously optimized antibody pairs
- Existing plate formats and incubation protocols
- Room temperature or automated diagnostic system conditions
Getting started
If your workflow uses streptavidin–biotin interactions in the capture step and you have been wondering how to access ultra-sensitive detection, the Exazym HRP Detection Kit is designed for exactly that scenario.
We are always interested in discussing specific assays and constraints. If you would like to explore whether the HRP kit makes sense for your work, reach out to our team to walk through your current setup and options.
Because breakthrough science should not require compromising on practicality.